A Cross Sectional Study on Prevalence of Antibiotic Resistance and Role of Efflux Pumps in Fluoroquinolone Resistance by using Efflux Pump Inhibitors in Isolated Cultures from Poultry, Dairy Farms and MTCC Strains from Reservoirs
Leena Seasotiya, Priyanka Siwach, Pooja Bharti, Sheema Bai, Anupma Malik, Ravinder Kumar, Sunita Dalal
Microbiology Research Journal International · pp. 107–116 · Published 11 Sep 2014
10.9734/BMRJ/2015/12342Abstract
Aims: Emergence of antibiotic resistance in bacterial strains has always remained a crucial concern. Mutations in antibiotic target sites, over expression of efflux pump are the major modes of development of bacterial antibiotic resistances. The present study was conducted to determine antibiotic resistance and role of efflux pumps in fluoroquinolone resistance by using efflux pump inhibitors. Place and Duration of Study: The Research was conducted during June 2011 to March 2012 at Department of Biotechnology, Kurukshetra University, Kurukshetra, Haryana – India. Methodology: Out of 57 bacterial strains 19 were procured from collection centres (reservoirs) and 38 were isolated from dairy (n = 10) and poultry farms (n = 28) and screened against 12 antibiotics of different groups by well assay. Further, fluoroquinolone sensitive/resistant strains were tested to observe the decline in minimum inhibitory concentration (MIC) levels in the presence/absence of efflux pump inhibitors. Results: Antibiotic resistance in tested strains was higher against nitrofurantoin, lincomycin, cefixime and chloramphenicol. Majority of the bacterial strains (94.74%) showed resistance to two or more antibiotics. Isolated bacterial strains were exhibiting more antibiotic resistance than reservoir strains indicating their exposure to antibiotics. Reduction in MIC (2-4 folds) was observed when Piperine (28.07%) or Plumbagin (19.29%) was used in combination with fluoroquinolones. The findings emphasized that majority of efflux pump inhibitors are active against Gram-positive bacteria. Overexpression of efflux pump and higher antibiotic resistance was also observed in subgroup III exhibiting resistance to combination of cefixime/nitrofurantoin. Conclusion: Efflux mediated resistance appears to contribute significantly to fluoroquinolone resistance and multidrug resistance in organisms, which may be due to involvement of active efflux pumps of both MFS and RND Family.
References (34)
- 1 Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically
- 2 Manual for the identification of medical bacteria.
- 3 Multidrug-resistance efflux pumps ? not just for resistance [DOI]
- 4 Medical Laboratory Manual for Tropical Countries
- 5 History of the Use of Antibiotic as Growth Promoters in European Poultry Feeds [DOI]
- 6 Vancomycin-Resistant Enterococci [DOI]
- 7 Methicillin resistance in staphylococci: molecular and biochemical basis and clinical implications [DOI]
- 8 The emergence and evolution of methicillin-resistant Staphylococcus aureus [DOI]
- 9 Experiments in Microbiology, Plant Pathology and Biotechnology
- 10 Bacterial resistance to macrolide, lincosamide, and streptogramin antibiotics by target modification [DOI]
- 11 Factors impacting on the problem of antibiotic resistance [DOI]
- 12 Bacterial efflux pump inhibitors from natural sources [DOI]
- 13 Evolutionary genomics of Staphylococcus aureus : Insights into the origin of methicillin-resistant strains and the toxic shock syndrome epidemic [DOI]
- 14 Efflux-mediated fluoroquinolone resistance in Staphylococcus aureus [DOI]
- 15 Mechanisms of Action and Resistance of Older and Newer Fluoroquinolones [DOI]
- 16 Evaluation of extracts of the root of Landolphia owerrience for antibacterial activity [DOI]
- 17 Efflux as a mechanism of resistance to antimicrobials in Pseudomonas aeruginosa and related bacteria: unanswered questions.
- 18 Piperine, a Phytochemical Potentiator of Ciprofloxacin against Staphylococcus aureus [DOI]
- 19 Aminoglycoside-modifying enzymes [DOI]
- 20 Prevalence and antimicrobial resistance of Salmonella isolated from lactating cows and in contact humans in dairy farms of Addis Ababa: a cross sectional study [DOI]
- 21 Bacterial Efflux Pumps Involved in Multidrug Resistance and their Inhibitors: Rejuvinating the Antimicrobial Chemotherapy [DOI]
- 22 Genetic Determinants of Antibiotic Resistance in Staphylococcus aureus Isolates from Milk of Mastitic Crossbred Cattle [DOI]
- 23 Quinolone resistance mediated by norA: physiologic characterization and relationship to flqB, a quinolone resistance locus on the Staphylococcus aureus chromosome [DOI]
- 24 Use of an Efflux Pump Inhibitor To Determine the Prevalence of Efflux Pump-Mediated Fluoroquinolone Resistance and Multidrug Resistance in Pseudomonas aeruginosa [DOI]
- 25 Importance of the Efflux Pump Systems in the Resistance of Mycobacterium tuberculosis to Fluoroquinolones and Linezolid [DOI]
- 26 Molecular Diagnostics of Medically Important Bacterial Infections [DOI]
- 27 Effect of efflux pump inhibitors on drug susceptibility of ofloxacin resistant Mycobacterium tuberculosis isolates.
- 28 High prevalence of antibiotic resistance in commensal Escherichia coli among children in rural Vietnam [DOI]
- 29 Molecular surveillance of putative virulence factors and antibiotic resistance in Staphylococcus aureus isolates recovered from intra-mammary infections of river buffaloes [DOI]
- 30 The multidrug efflux transporter of Bacillus subtilis is a structural and functional homolog of the Staphylococcus NorA protein [DOI]
- 31 Multiple mutations conferring ciprofloxacin resistance in Staphylococcus aureus demonstrate long-term stability in an antibiotic-free environment [DOI]
- 32 Horizontal Gene Transfers Link a Human MRSA Pathogen to Contagious Bovine Mastitis Bacteria [DOI]
- 33 Use of resazurin to detect mefloquine as an efflux-pump inhibitor in Pseudomonas aeruginosa and Escherichia coli [DOI]
- 34 Synthesis and evaluation of inhibitors of bacterial drug efflux pumps of the major facilitator superfamily [DOI]
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