A 100% Water Mobile Phase HPLC-PDA Analysis of Tetracycline Antibiotics
Chemical Science International Journal · pp. 500–506 · Published 27 Aug 2013
10.9734/ACSJ/2013/3868Abstract
Aims: To develop a reserved-phase HPLC method for detecting oxytetracycline (OTC), tetracycline (TC), and chlortetracycline (CTC) using a 100% water mobile phase. Study Design: HPLC conditions. Place and Duration of Study: Author’s Lab., Osaka City University, Japan, between September and November 2012. Methodology: Chromatographic separations were performed an Inertsil® WP300 C4 (100×4.6 mm, 5 μm) with a water mobile phase and a photodiode-array detector. Results: The run time was < 5.5 min. The method shows high stability, significant linearity and satisfactory sensitivity. The detection limits were established in the range 0.01 – 0.059 μg/mL. Conclusion: An organic solvent/reagent-free HPLC method for the simultaneous detection of OTC, TC, and CTC was developed and may be further applied to the quantification in foods.
References (14)
- 1 Green Chemistry: Theory and Practice
- 2 Chromatographic analysis of tetracycline antibiotics in foods [DOI]
- 3 A HPLC with fluorescence detection method for the determination of tetracyclines residues and evaluation of their stability in honey [DOI]
- 4 Multi-residue determination of seventeen sulfonamides and five tetracyclines in fish tissue using a multi-stage LC–ESI–MS/MS approach based on advanced mass spectrometric techniques [DOI]
- 5 Simultaneous residue monitoring of four tetracycline antibiotics in fish muscle by in-tube solid-phase microextraction coupled with high-performance liquid chromatography [DOI]
- 6 Validation of Chromatographic Methods [DOI]
- 7 A confirmatory method for the determination of tetracyclines in muscle using high-performance liquid chromatography with diode-array detection [DOI]
- 8 Determination of tetracycline residues in chicken meat by liquid chromatography-tandem mass spectrometry [DOI]
- 9 Development and Validation of a Liquid Chromatographic/Tandem Mass Spectrometric Method for Determination of Chlortetracycline, Oxytetracycline, Tetracycline, and Doxycycline in Animal Feeds [DOI]
- 10 Isolation of tetracyclines in milk using a solid-phase extracting column and water eluent [DOI]
- 11 Stability studies of vorinostat and its two metabolites in human plasma, serum and urine [DOI]
- 12 Multiclass, multiresidue method for the detection of antibiotic residues in distillers grains by liquid chromatography and ion trap tandem mass spectrometry [DOI]
- 13 Liquid Chromatographic Determination of Oxytetracycline in Edible Fish Fillets from Six Species of Fish [DOI]
- 14 On-line dialysis, liquid chromatography and post-column reaction detection of oxytetracycline in salmon muscle extracts [DOI]
Cited by 3
Rúbia Adrieli Sversut, Adriane Andrade da Silva, Teófilo Fernando Mazon Cardoso · Critical Reviews in Analytical Chemistry · 2016
Roaa M. Khaleel, Dawooh H. Mohammed · Journal of Physics: Conference Series · 2020
Szymon Bocian, Katarzyna Krzemińska · Green Chemistry Letters and Reviews · 2019
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