Enhancing Mungbean Resistance to Yellow Mosaic Virus: Insights from Biochemical Studies

Abstract

Mungbean is one among the important and major pulse crop for supplementation of protein in subtropical zones of the world. Apart from providing protein in the diet, it also has the appreciable quality of helping the symbiotic root rhizobia by contributing to fix atmospheric nitrogen enriches the fertility of soil. Among the biotic factors, Mungbean yellow mosaic virus (MYMV) is the major bottleneck for pulse producers. The current study is aimed at estimation of biochemical enzymes like peroxidase, polyphenol oxidase, catalase, phenols and sugars contributing for resistance in mungbean genotypes. Fourteen Mungban genotypes including one susceptible check MYB-11 and KKM-3 (UASB released variety) were screened during kharif 2022 and summer 2023. To determine MYMV induced biochemical changes in mungbean, the infected leaf samples were collected from resistant, moderately resistant, moderately susceptible and susceptible genotypes. The varied level of different defense inducing enzymes from all the 14 mungbean genotypes was estimated. The biochemical constituents such as total sugar, total phenol content and enzyme activity of Peroxidase (PO), Polyphenol oxidase (PPO) and catalase activity were estimated. The present study indicated that the defence inducing enzymes, i.e, peroxidase, polyphenol oxidase, catalase, phenols and sugars, showed comparatively higher level of resistance in genotypes viz., MYB- 6, 7, 8 and 9 when compared to the susceptible check (MYB-11).