Determination of Vitamin B6, B9 and B12 in Halophytes by Solid Phase Extraction Followed by HPLC-UV
Narges Chamkouri, Mona Vafaeizadeh, Ayyub Mojaddami, Seyyed Abed Hosseini, Tahereh Momeni Isfahani, Aida Ghorbani Golkhajeh, Milad Janghorban Lariche, Seyed Mohammad Ali Malaekeh
Journal of Pharmaceutical Research International · pp. 1–6 · Published 8 Jan 2018
10.9734/JPRI/2017/34730Abstract
In this work, solid phase extraction (SPE) was proposed for simultaneous determination of trace levels of vitamin B6, B9 and B12. High performance liquid chromatography with UV detection (HPLC-UV) was used. The experimental conditions including pH of sample and type and volume of extraction solvent were studied by one-factor-at-a time method. The limit of detection in the optimal conditions was 14.91, 10.03, and 7.25 ngkg-1 for vitamins B6, B9 and B12 respectively and relative standard deviations (RSD) of the analysis less than 8% (n= 3). Recoveries of vitamins B6, B9 and B12 were in the ranges of 80–93%. The proposed method was successfully applied for the simultaneous determination of vitamin B6, B9 and B12 in halophyte samples such as, Salicornia, Suaeda monoica, Suaeda aegyptiaca, Sueada vera and Sueada martima.
Cited by 2
Joanna Żandarek, Żaneta Binert-Kusztal, Małgorzata Starek · Processes · 2023
Zakia Fatima, Xiangzi Jin, Yilin Zou · Journal of Chromatography A · 2019
Related research
- Behaviour of Salicornia neei Cultivated in Different Spacing and Irrigated with Desalinizer Tailings — shares topic coverage
- Amino Acid Patterns in the Aerial Parts of Echium L. and Anchusa L. Growing in the Sand Dunes of Turkey — shares topic coverage
- In vitro Pepper (Capsicum annuum L.) Anther Culture: Can be Affected Via Vitamins B? — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
2
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.