Identification of Resistance Gene for Mungbean Yellow Mosaic Virus (MYMV) in Resistant Mungbean Genotypes through RGA Markers

Abstract

Mungbean is one among the important and major pulse crop for supplementation of protein in subtropical zones of the world. The yield of mungbean is affected by both abiotic and biotic factors. Among the biotic factors, Mungbean yellow mosaic virus (MYMV) is the major bottleneck for pulse producers. MYMV cause a Yellow mosaic disease and its transmitted by Aleyrodidae insects. The virus belong to the family Geminiviridae and the genus Begomovirus. Fourteen mungbean genotypes procured from Asian Vegetable Research and Development Centre (AVRDC), Taiwan were screened for Mung bean Yellow Mosaic Virus resistance in field and laboratory conditions during Kharif 2022 and summer 2023. Genotypes MYB-6, 7, 8, 9 and 12 were found to be resistant under field conditions by less PDI and AUDPC values. The molecular confirmation of MYMV resistance was tested using Resistant Gene Analog (RGA) in all 14 genotypes. The RGA was amplified at 90bp in resistant genotypes viz MYB-6, 7, 8 and 9. Since MYMV resistant lines MYB-6, 7, 8 and 9 showed resistance in both field and molecular studies, hence these lines can be used as a YMV donor in breeding process and also can be released as a variety after conducting a multi-location trails in MYMV hotspot conditions.