16α-Hydroxycholic Acid: In Vivo Microbial Transformation Product of Cholic Acid
Samira Al-Awadhi, Sosamma Oommen, Mohammad Afzal
Journal of Pharmaceutical Research International · pp. 374–390 · Published 12 Apr 2013
10.9734/BJPR/2013/3194Abstract
Aims: This study describes the transformation of cholic acid to hydroxylated cholic acid metabolites that could not be easily synthesized. Study Design: The transformation was catalyzed by thermophilic Geobacillus stearothermophilus comb. nov., isolated from oil contaminated soil in Kuwait. Cholic acid, as the sole source of carbon, was added to G. stearothermophilus cells in phosphate buffer pH 7 and shaken at 65ºC for 5 days. Methodology: The cholic acid transformation products were extracted with ethyl acetate, purified on preparative TLC plates and their molecular structures were established from their spectral data. Results: The bacterium could selectively oxidize hydroxyl-groups at C3 and C7 while leaving the C12-hydroxyl-group unoxidized, in cholic acid. Five commonly found metabolites of cholic acid and a novel transformation product, 16α-hydroxycholic acid, were identified. Conclusion: Our results indicate that G. stearothermophilus can hydroxylate/oxidize a steroid nucleus at various ring positions, and has a unique ability for hydroxylation at C16α in cholic acid.
Cited by 1
Tang Shijing, Pan Yinping, Yang Qiong · Microbial Cell Factories · 2024
Related research
- Triterpenes and Triterpenoids Clinically Useful with Multiple Targets in Cancer, Malaria and More Treatment: Focus on Potential Therapeutic Value — shares topic coverage
- Screening and Identification of Lactic Acid Bacteria with D-tagatose Production Capability — shares topic coverage
- Impact of Agrochemicals on Soil Biota and Ways to Mitigate it: A Review — shares topic coverage
- Studying the Possible Biotransformation of the Cytotoxic Diterpenoid Paclitaxel Using Jatropha Curcas Cell Suspension Culture — shares topic coverage
- Biotransformation of Unsold Mangoes and Cashew Apples by Acetic Acid Bacteria Isolated from Fermented Beverages — shares topic coverage
Article metrics
Real usage data collected on this platform.
1
Page views
0
PDF downloads
0
Outbound clicks
1
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.