Isolation and Identification of Bacteria Associated with Aerial Part of Rice Plant from Kware Lake

Abstract

A study was conducted to isolate, identify and establish the Pathogenecity test of bacteria associated with diseases affecting the aerial part of rice in kware lake. Bacteria was isolated from the infected parts collected from Kware Lake and identified by microscopy and biochemical tests. The isolated bacterial were Staphylococcus aureus, Xanthomonas oryzae, Staphylococcus intermedius, Clostridium noryi, Pseudomonas putida, Corynoebacterium xerosis, and Bacillus macerans. However, Staphylococcus aureus, occurred more frequently with 32.93%, followed by Xanthomonas oryzae 27.44%, Corynoebacterium xerosis 20.73%, then Staphylococcus intermedius 14.63%, Baccilus macerans 2.44%, Clostridium noryi 1.22% and Pseudomonas putida with 0.61%. The biochemical reactions include: citrate test, spore test, indole test, starch hydrolysis, urease test, catalyse test, gas formation, glucose, sucrose and lactose test, motility test, H2S production and gram reactions, but only two of the identified bacteria caused infection to the plant after undergoing pathogenecity test. They are therefore recommended for the possible infection of the aerial part of rice caused by these pathogenic bacteria.