Tuna Bone Waste (Thunnus sp.) as a Hydroxyapatite Source: Synthesis and Characterization for Biomaterial Applications
Asian Journal of Advanced Research and Reports · pp. 152–158 · Published 9 Aug 2025
10.9734/ajarr/2025/v19i81121Abstract
Tuna fish bone waste, a by-product of fish processing, exhibits promising potential for conversion into hydroxyapatite (HA), a high-value biomaterial with medical applications. This study successfully synthesized HA from tuna bone waste through a combined approach of calcination (900°C, 10 hours) and wet precipitation using (NH₄)₂HPO₄ at a Ca/P ratio of 1.67. The process achieved a conversion efficiency of 33.97%, producing HA with 81.7% phase purity along with a secondary whitlockite phase (18.3%) attributed to low pH conditions and Mg²⁺ ion presence. Comprehensive characterization revealed: (1) a hexagonal HA crystal structure with lattice parameters a = 9.424 Å and c = 6.879 Å (XRD analysis), and (2) spherical small particles (200-1000 nm) displaying agglomeration tendencies (SEM observation). The synthesized material demonstrates suitable characteristics for biomedical applications including bone grafts and tissue engineering scaffolds. This research presents an eco-friendly strategy for valorizing fishery waste while decreasing reliance on costly synthetic HA sources. Future studies should focus on optimizing the synthesis process to enhance both yield and purity.
References (4)
- 1 Fishery Wastes as a Yet Undiscovered Treasure from the Sea: Biomolecules Sources, Extraction Methods and Valorization [DOI]
- 2 Resorbable Mg2+-Containing Phosphates for Bone Tissue Repair [DOI]
- 3 Spontaneous Osteogenic Differentiation of Human Mesenchymal Stem Cells by Tuna-Bone-Derived Hydroxyapatite Composites with Green Tea Polyphenol-Reduced Graphene Oxide [DOI]
- 4 Morphology and particle size analysis of hydroxyapatite micro- and nano-particles [DOI]
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