Prevalence of Escherichia coli Pathotypes among Children with Diarrhoea in Zaria, Nigeria
A. Sani, J. A. Onaolapo, Y. K. E. Ibrahim, H. W. Idris, J. C. Igwe, A. Nworie
Journal of Advances in Medicine and Medical Research · pp. 17–24 · Published 29 Jan 2015
10.9734/BJMMR/2015/15623Abstract
Diarrhoea is one of the foremost public health problems worldwide especially among Children under five years in developing countries. Only few studies have investigated the epidemiology and virulence of Escherichia coli pathotypes in South-Eastern and South-Western Nigeria leaving the Northern part of the country unstudied. In this study, a total of 100 isolates of E. coli (45%) were obtained from the stool of 222 diarrhoea patients who were children below five (5) years attending Ahmadu Bello University Teaching Hospital, Shika, and Institute of Child Health, Banzazzau; an annex of Ahmadu Bello University Teaching Hospital, Shika-Zaria, Nigeria. The isolation and biochemical identification of the E. coli isolates were performed using standard microbiological procedures. A multiplex polymerase chain reaction (PCR) technique was used to differentiate the five (5) major diarrhoeagenic Escherichia coli pathotypes (EHEC, ETEC, EPEC, EIEC and EAEC) in one reaction condition, by using different diarrhoeagenic E. coli primers for different virulent genes found in E. coli. From the result obtained, only one (1) percent of the isolates was found to harbor the virulence gene out of the 100 E. coli isolated from the diarrhoea stools of children employed in this study.
References (15)
- 1 District Laboratory Practice in Tropical Countries [DOI]
- 2 The magnitude of the global problem of diarrhoeal disease: a ten-year update.
- 3 Case-Control Study of Enteropathogens Associated with Childhood Diarrhea in Dhaka, Bangladesh [DOI]
- 4 Aggregative adherence fimbriae I of enteroaggregative Escherichia coli mediate adherence to HEp-2 cells and hemagglutination of human erythrocytes [DOI]
- 5 Malaria: [DOI]
- 6 Multidrug Resistance and Plasmid Patterns of Escherichia coli O157 and Other E. coli Isolated from Diarrhoeal Stools and Surface Waters from Some Selected Sources in Zaria, Nigeria [DOI]
- 7 ETIOLOGY OF DIARRHEA IN CHILDREN LESS THAN FIVE YEARS OF AGE IN IFAKARA, TANZANIA [DOI]
- 8 A study of the aetiological agents of childhood diarrhoea in Lagos, Nigeria [DOI]
- 9 Diarrhoeagenic Escherichia coli [DOI]
- 10 A method for fast and simple detection of major diarrhoeagenic Escherichia coli in the routine diagnostic laboratory [DOI]
- 11 Distribution and expression of the astA gene (EAST1 toxin) in Escherichia coli and Salmonella [DOI]
- 12 Etiological agents of infectious diarrhea: implications for requests for microbial culture [DOI]
- 13 Hemolytic-uremic syndrome and enterohemorrhagic Escherichia coli [DOI]
- 14 Virulence properties of diarrheagenic E. coli and etiology of diarrhea in infants, young children and other age groups in Southeast, Nigeria.
- 15 Characterization of Diarrhoeagenic Escherichia coli isolates in Jordanian Children [DOI]
Cited by 3
R. H. Abimiku, Y. Ngwai, I. Nkene · International Journal of Pathogen Research · 2019
Nfongeh Joseph Fuh, Owoseni Mojisola Christiana, Upla Peter Uteh · 2018
Related research
- Antibacterial Activity of Bidens pilosa Extracts on Escherichia coli O157: H7 Isolated from Apparently Healthy Individuals — shares topic coverage
- Urine Analysis and Determination of Drug Sensitivity Pattern of Isolated Escherichia coli from Urinary Tract Infected Patients — shares topic coverage
- Evaluation of the Effects of Pediococcus acidilactici Isolated from Wara, a Nigerian Milk Product, in the Prevention of Diarrhea and the Modulation of Intestinal Microflora in Wistar Rats — shares topic coverage
- Prevalence, Aetiological Agents and Antimicrobial Resistance Patterns of Urinary Tract Infections among Pregnant Women Attending Mother and Child Hospital, Akure, South-West Nigeria — shares topic coverage
- Molecular Detection of the Arr-2 Gene in Escherichia coli and Klebsiella pneumoniae Resistant to Rifampicin in Abidjan, Côte D'Ivoire — shares topic coverage
Article metrics
Real usage data collected on this platform.
66
Page views
0
PDF downloads
36
Outbound clicks
3
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.