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Research Article Open access CC BY 4.0

Postmortem Diagnosis of Induced Fatal Anaphylactic Shock in Rats

Said Said Elshama, Rasha R. Salem, Hosam-Eldin Hussein Osman, Ayman El-Meghawry El-Kenawy

Annual Research & Review in Biology · pp. 1–14 · Published 5 Feb 2018

10.9734/ARRB/2018/39315

Abstract

Anaphylactic shock is a sudden and serious life-threatening systemic hypersensitivity leading to a rapid, irreversible fatal circulatory collapse. Postmortem diagnosis of fatal anaphylaxis is a very sophisticated task in forensic medicine; it is usually excluded as the cause of death due to lack of autopsy findings. This study aims to find more specific criteria for the postmortem diagnosis of induced fatal anaphylaxis in rats by assessing the levels of total tryptase, histamine, immunoglobulin E (IgE) and histological changes in the larynx, trachea, lung, heart, and spleen using light and electron microscopes. Sixty adult albino rats were divided into three groups; each group consisted of twenty rats. The first (control) group received distilled water while the second and third groups received a single intravenous dose of ovalbumin and penicillin G, respectively, two weeks after active subcutaneous sensitization. The fatal anaphylactic shock led to a significant increase in the levels of total tryptase, histamine and immunoglobulin E (IgE) along with histological changes in the larynx, trachea, lung, heart, and spleen that vary its severity according to the anaphylaxis cause. Postmortem diagnosis of fatal anaphylaxis depends on multi- factorial criteria that include biochemical, immunological and histological findings.

Diagnosis anaphylaxis postmortem

References (21)

  1. 1 The diagnosis and management of anaphylaxis practice parameter: 2010 Update [DOI]
  2. 2 Diagnostic Value of Tryptase in Anaphylaxis and Mastocytosis [DOI]
  3. 3 Postmortem findings after fatal anaphylactic reactions: Table 1 [DOI]
  4. 4 Anaphylaxis: Clinical patterns, mediator release, and severity [DOI]
  5. 5 Development of a new, more sensitive immunoassay for human tryptase: Use in systemic anaphylaxis [DOI]
  6. 6 Fatal anaphylaxis: postmortem findings and associated comorbid diseases [DOI]
  7. 7 Correlation of serum allergy (IgE) tests performed by different assay systems [DOI]
  8. 8 Tryptase genetics and anaphylaxis [DOI]
  9. 9 Mast cell tryptase in postmortem serum—reference values and confounders [DOI]
  10. 10 Anaphylactic deaths in Auckland, New Zealand: a review of coronial autopsies from 1985 to 2005 [DOI]
  11. 11 Utilization of Serum Tryptase and Immunoglobulin E Assay in the Postmortem Diagnosis of Anaphylaxis [DOI]
  12. 12 Anaphylaxis pathogenesis and treatment [DOI]
  13. 13 Hypersensitivity reactions to drugs: evaluation and management.
  14. 14 Hepatic venoconstriction is involved in anaphylactic hypotension in rats [DOI]
  15. 15 Postmortem diagnosis of fatal anaphylaxis during intravenous administration of therapeutic and diagnostic agents: Evaluation of clinical laboratory parameters and immunohistochemistry in three cases [DOI]
  16. 16 Postmortem Serum Levels of Tryptase and Total and Specific IgE in Fatal Asthma [DOI]
  17. 17 Laboratory diagnosis of acute anaphylaxis [DOI]
  18. 18 A rat model of Shuang Huang Lian injection-induced anaphylaxis.
  19. 19 Evaluation of a radioimmunoassay for histamine measurement in biologic fluids [DOI]
  20. 20 The detection of fatal anaphylaxis at autopsy - an overview
  21. 21 Cross reactivity of cephalosporins with penicillin.

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