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Research Article Open access CC BY 3.0

Phytochemical Screening and Biological Activities of Hypotrigona squamuligera Raw Honey

P. Dzomba, N. Ngoroyemoto, L. Mutandwa, D. Shasha

International Journal of Biochemistry Research & Review · pp. 98–105 · Published 5 Sep 2012

10.9734/ijbcrr/2012/1562

Abstract

Aims: To evaluate phytochemical content and biological activities of Hypotrigona squamuligera honey. Study Design: Standard chemical tests, DPPH antiradical activity assay and agar diffusion methods. Place and Duration of Study: Department of Chemistry (Natural product section) and Biological Science Department November 2011 and March 2012. Methodology: Standard chemical tests, DPPH antiradical activity and agar diffusion methods were adopted to determine phytochemical compounds present, antioxidant and antibacterial activity respectively. The following bacterial strains were employed in the screening studies: Staphylococcus aureus and Escherichia coli, all from stock cultures of Bindura University Biological Sciences Department. Results: Phytochemical screening revealed presence of tannins, alkaloids, flavonoids, terpenoids, anthraquinones and cardiac glycosides. H. squamuligera honey exhibited marked antioxidant and antibacterial activity. Its antiradical activity was lower than that of ascorbic acid and quercetin at low concentrations 0,0125 and 0,025mg/ml however as from 0,05mg/ml methanolic extract exhibited significantly higher antioxidant activity. H. squamuligera honey (100%) produced a greater inhibition (24 ± 0,09 mm) than amoxicillin (19±0,01mm) for S. aureus. For E. coli amoxicillin produced greater inhibition than H. squamuligera honey. Compared to Apis mellifera honey at a concentration of 100%, H. squamuligera honey revealed better inhibitory activity. Minimum inhibitory values ranged from 10 - 25 μg/ml. The lowest MIC value (15μg/ml) for H. squamuligera honey was recorded for S. aureus. Conclusion: The results suggest that H. squamuligera honey could potentially be used as an alternative pharmaceutical agent against certain microorganisms and source of natural antioxidants for promotion of health however there is a need to perform structural elucidation to identify the structures of phytochemical compounds present in the honey.

Hypotrigona squamuligera phytochemicals antioxidant activity antibacterial activity

References (21)

  1. 1 Antioxidant Activity of Plant Extracts Containing Phenolic Compounds [DOI]
  2. 2 Burn Wound Infections [DOI]
  3. 3 Identification and quantification of methylglyoxal as the dominant antibacterial constituent of Manuka ( Leptospermum scoparium) honeys from New Zealand [DOI]
  4. 4 Antioxidant Capacity of Honeys from Various Floral Sources Based on the Determination of Oxygen Radical Absorbance Capacity and Inhibition of in Vitro Lipoprotein Oxidation in Human Serum Samples [DOI]
  5. 5 Bactericidal Activity of Different Honeys against Pathogenic Bacteria [DOI]
  6. 6 Mechanism of Synergy between Epigallocatechin Gallate and β-Lactams against Methicillin-Resistant Staphylococcus aureus [DOI]
  7. 7 Inhibitory activity of honey against foodborne pathogens as influenced by the presence of hydrogen peroxide and level of antioxidant power [DOI]
  8. 8 Evaluation of antioxidant activity and total phenolics of selected Czech honeys [DOI]
  9. 9 Screening the antioxidant and antimicrobial properties of the lichens Parmelia saxatilis, Platismatia glauca, Ramalina pollinaria, Ramalina polymorpha and Umbilicaria nylanderiana [DOI]
  10. 10 Antifungal activity of lichen extracts and lichenic acids [DOI]
  11. 11 Stenotrophomonas maltophilia resistant to trimethoprim-sulfamethoxazole: an increasing problem. [DOI]
  12. 12 The antimicrobial activity of substances derived from the lichens Physcia aipolia, Umbilicaria polyphylla, Parmelia caperata and Hypogymnia physodes [DOI]
  13. 13 Total Antioxidant Content of Alternatives to Refined Sugar [DOI]
  14. 14 Taxonomic revision of the African stingless bees (Apoidea : Apidae : Apinae : Meliponini)
  15. 15 Antimicrobial activity of two South African honeys produced from indigenous Leucospermum cordifolium and Erica species on selected micro-organisms [DOI]
  16. 16 ANTIBACTERIAL ACTIVITY OF LOCAL MALAYSIAN HONEY
  17. 17 Chemical profile and antimicrobial activity of Boldo (Peumus boldus Molina) extracts obtained by compressed carbon dioxide extraction [DOI]
  18. 18 The potential for honey in the management of wounds and infection [DOI]
  19. 19 Potential of Malaysian local honey as an antibacterial agent
  20. 20 Nutraceuticals and functional foods: I. Current trend in phytochemical antioxidant research
  21. 21 Contents of major phenolic and flavonoid antioxidants in selected Czech honey [DOI]

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