Physico-Chemical Properties of Purified Carboxylesterase from the Seeds of Tamarindus indica
Asian Journal of Research in Biochemistry · pp. 42–58 · Published 6 Feb 2021
10.9734/ajrb/2021/v8i130173Abstract
The present work is focus on physical and chemical properties of purified Carboxylesterase using the Seeds of Tamarindus Indica.The esterases are extracted from the germinating tamarind seeds using 50 mM phosphate buffer, pH 7 and purified. The Km with α-naphthyl acetate, β-naphthyl acetate and α-naphthyl butyrate as the substrates is 28.6 μM, 22.2 μM and 26.7 μM respectively. The Vmax for the same substrates is 7.1 x 10-3 µmole/min, 7.41 x 10-3 µmole/min and 8.00 x 10-3 µmole/min respectively. The enzymes optimally active at pH 7.0 and are stable between pH 5.0 to 8.0. The optimum temperature of esterase activity is 40˚C. The molecular weight of 27.5 kD as determined by SDS-PAGE, both in the presence and absence of β-mercaptothanol and is in close agreement with the molecular weight determined by gel-filtration on Sephadex G-100 (26.9 kD).
References (34)
- 1 THE MAMMALIAN CARBOXYLESTERASES: From Molecules to Functions [DOI]
- 2 Serum esterases. 2. An enzyme hydrolysing diethyl p-nitrophenyl phosphate (E 600) and its identity with the A-esterase of mammalian sera [DOI]
- 3 Keynote review: Mammalian carboxylesterases: From drug targets to protein therapeutics [DOI]
- 4 Hydrolysis of pyrethroids by human and rat tissues: Examination of intestinal, liver and serum carboxylesterases [DOI]
- 5 Individual variability in esterase activity and CYP1A levels in Chinook salmon (Oncorhynchus tshawytscha) exposed to esfenvalerate and chlorpyrifos [DOI]
- 6 Comparing the organophosphorus and carbamate insecticide resistance mutations in cholin- and carboxyl-esterases [DOI]
- 7 Purification and cloning of an esterase from the weed black‐grass (Alopecurus myosuroides), which bioactivates aryloxyphenoxypropionate herbicides [DOI]
- 8 SDS Polyacrylamide Gel Electrophoresis of Proteins [DOI]
- 9 Role of detoxication pathways in acute toxicity levels of phosphorothionate insecticides in the rat [DOI]
- 10 Enzyme inhibition by fluoro compounds. [DOI]
- 11 Carboxylesterases, importance for detoxification of organophosphorus anticholinesterases and trichothecenes [DOI]
- 12 Purification and characterization of a heat-stable esterase from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius [DOI]
- 13 Biochemical characterisation of esterases active in hydrolysing xenobiotics in wheat and competing weeds [DOI]
- 14 Carboxylesterases from the seeds of an underutilized legume, Mucuna pruriens; isolation, purification and characterization [DOI]
- 15 Catalytic Properties of an Unspecific Carboxylesterase (E1) from Rat‐Liver Microsomes [DOI]
- 16 Plant biochemistry of xenobiotics. Purification and properties of a wheat esterase hydrolyzing the plasticizer chemical, bis(2-ethylhexyl)phthalate [DOI]
- 17 Purification and properties of carboxylesterase B of Escherichia coli [DOI]
- 18 Carboxylic Ester Hydrolases of Apple [DOI]
- 19 Purification and characterization of a carboxylesterase from the intestine of the nematode Caenorhabditis elegans [DOI]
- 20 Purification and characterisation of a carboxylesterase from the latex ofSynadenium grantii Hook, ‘f’ [DOI]
- 21 Purification and properties of an esterase from Cucurbita maxima fruit tissue [DOI]
- 22 Different forms of pig liver esterase [DOI]
- 23 Carboxylesterases (EC 3.1.1). Purification and Titration of Chicken, Sheep, and Horse Liver Carboxylesterases [DOI]
- 24 Purification and characterization of carboxylesterases from rat lung [DOI]
- 25 ESTERASE ACTIVITY FROM THE GERMINATED JATROPHA CURCAS SEEDS IN DIFFERENT EXTRACTION BUFFERS
- 26 Isolation, Properties, and the Complete Amino Acid Sequence of a Second Form of 60-kDa Glycoprotein Esterase [DOI]
- 27 Differences in Hydrolysis and Binding of Homologous Juvenile Hormones in Locusta migratoria Hemolymph [DOI]
- 28 [24] Carboxylesterases from pig and ox liver [DOI]
- 29 Identification of the chloramphenicol-hydrolyzing enzyme of guinea pig liver as one of the nonspecific carboxylesterases [DOI]
- 30 Nonspecific esterase isozyme polymorphism in natural populations of Gossypium thurberi [DOI]
- 31 Isolation and properties of a carboxylesterase from pig kidney [DOI]
- 32 Identification and isolation of a unique esterase from the medium of non-embryogenic cell line of cultured carrot cells [DOI]
- 33 Carboxylesterases, Importance for Detoxification of Organophosphorus Anticholinesterases and Trichothecenes [DOI]
- 34 Chemical Pesticides Mode of Action and Toxicology [DOI]
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