Skip to content
Research Article Open access CC BY 3.0

Maternal Obesity and Malnutrition in Rats Differentially Affect Glucose Sensing in the Muscles and Adipose Tissues in the Offspring

Maher A. Kamel, Madiha H. Helmy, Mervat Y. Hanafi, Shimaa A. Mahmoud, Hanan Abo Elfetooh, Mahmoud S. Badr

International Journal of Biochemistry Research & Review · pp. 440–469 · Published 29 May 2014

10.9734/IJBCRR/2014/10649

Abstract

Background: The altered maternal/fetal metabolism appears to be associated with a diabetogenic effect in the adult offspring even in the absence of genetic predisposition. Aim: The study aimed to investigate the effect of maternal obesity and malnutrition on the peripheral glucose sensing and mitochondria biogenesis in F1 offspring. Effect of postnatal diet was also evaluated.   Methods: Three groups of female Wistar rats were used (control, obese and malnourished). After the pregnancy and delivery the offspring were weaned to control diet or high-caloric (HCD) diet and followed up for 30 weeks. Every 5 weeks OGTT was constructed and serum and tissues were obtained for assessment of glucose homeostasis parameters, mTFA, mtDNA, UCP2, insulin receptor (IR), phospho-insulin receptor (Phosho-IR) and GLUT4. Results: The results indicated that maternal obesity impair glucose tolerance and sensing in the offspring from the 15th week of age even under control diet and the situation is worse under HCD these defects were preceded by significant decline in mtDNA copy number in muscle and adipose tissues as early as 5th week of age. The offspring of malnourished mothers show normal and even better glucose tolerance and sensing and normal mtDNA copy number under control diet, while those offspring under HCD show impaired glucose sensing and tolerance only at older age than obese group.    Conclusion: maternal obesity and malnutrition differentially affect glucose sensing and tolerance, mtDNA copy number and the expression of genes involved in the mitochondrial biogenesis and function in the muscles and adipose tissues in the F1 offspring with the postnatal feeding appearing to play a central role in these differential effects. The male F1 offspring appear to be more sensitive for fetal diabetogenic programming than female offspring.

Fetal origin diabetes epigenetic glucose sensing insulin resistance UCP2 mitochondria

References (43)

  1. 1 Developmental Origins of the Metabolic Syndrome: Prediction, Plasticity, and Programming [DOI]
  2. 2 Developmental plasticity and human health [DOI]
  3. 3 Diet-Induced Obesity in Female Mice Leads to Offspring Hyperphagia, Adiposity, Hypertension, and Insulin Resistance [DOI]
  4. 4 Fetal Origins of Obesity [DOI]
  5. 5 Early origins of cardiovascular disease: is there a unifying hypothesis? [DOI]
  6. 6 Fetuses of Obese Mothers Develop Insulin Resistance in Utero [DOI]
  7. 7 Effect of a Low Protein Diet during Pregnancy on the Fetal Rat Endocrine Pancreas [DOI]
  8. 8 The Role of Mitochondria in the Pathogenesis of Type 2 Diabetes [DOI]
  9. 9 Impact of maternal obesity on offspring obesity and cardiometabolic disease risk [DOI]
  10. 10 Molecular Basis for the Dephosphorylation of the Activation Segment of the Insulin Receptor by Protein Tyrosine Phosphatase 1B [DOI]
  11. 11 Maternal obesity at conception programs obesity in the offspring [DOI]
  12. 12 Glucosensing and glucose homeostasis: From fish to mammals [DOI]
  13. 13 Prenatal Stress or High-Fat Diet Increases Susceptibility to Diet-Induced Obesity in Rat Offspring [DOI]
  14. 14 Mitochondrial Transcription Factor A Induction by Redox Activation of Nuclear Respiratory Factor 1 [DOI]
  15. 15 Impact of Nutrition on Oocyte Quality: Cumulative Effects of Body Composition and Diet Leading to Hyperinsulinemia in Cattle1 [DOI]
  16. 16 Offspring from mothers fed a ‘junk food’ diet in pregnancy and lactation exhibit exacerbated adiposity that is more pronounced in females [DOI]
  17. 17 Maternal obesity accelerates fetal pancreatic β-cell but not α-cell development in sheep: prenatal consequences [DOI]
  18. 18 Intrauterine growth restriction and adult disease: the role of adipocytokines [DOI]
  19. 19 Current Thoughts on Maternal Nutrition and Fetal Programming of the Metabolic Syndrome [DOI]
  20. 20 Up-Regulation of Toll-Like Receptor 4/Nuclear Factor-κB Signaling Is Associated with Enhanced Adipogenesis and Insulin Resistance in Fetal Skeletal Muscle of Obese Sheep at Late Gestation [DOI]
  21. 21 Fetal origins of adult diabetes [DOI]
  22. 22 Critical differences between two low protein diet protocols in the programming of hypertension in the rat [DOI]
  23. 23 Thiazolidinedione Treatment Normalizes Insulin Resistance and Ischemic Injury in the Zucker Fatty Rat Heart [DOI]
  24. 24 Brief Undernutrition in Late-Gestation Sheep Programs the Hypothalamic-Pituitary-Adrenal Axis in Adult Offspring [DOI]
  25. 25 Molecular mechanisms of lipid‐induced insulin resistance in muscle, liver and vasculature [DOI]
  26. 26 Initial steps of insulin signaling and glucose transport are defective in the type 2 diabetic rat heart [DOI]
  27. 27 Fetal and Early Postnatal Protein Malnutrition Cause Long-Term Changes in Rat Liver and Muscle Mitochondria [DOI]
  28. 28 Mitochondria and endocrine function of adipose tissue [DOI]
  29. 29 Accelerated Postnatal Growth Increases Lipogenic Gene Expression and Adipocyte Size in Low–Birth Weight Mice [DOI]
  30. 30 Uncoupling protein-2 attenuates glucose-stimulated insulin secretion in INS-1E insulinoma cells by lowering mitochondrial reactive oxygen species [DOI]
  31. 31 Evidence that a maternal “junk food” diet during pregnancy and lactation can reduce muscle force in offspring [DOI]
  32. 32 Fetal origins of insulin resistance and the metabolic syndrome: A key role for adipose tissue? [DOI]
  33. 33 Mechanisms of developmental programming of the metabolic syndrome and related disorders [DOI]
  34. 34 Fetal Programming of Diabetes: Still So Much to Learn! [DOI]
  35. 35 Insulin-induced receptor loss in cultured human lymphocytes is due to accelerated receptor degradation. [DOI]
  36. 36 Simultaneous A8344G heteroplasmy and mitochondrial DNA copy number quantification in Myoclonus Epilepsy and Ragged-Red Fibers (MERRF) syndrome by a multiplex Molecular Beacon based real-time fluorescence PCR [DOI]
  37. 37 Up-regulation of muscle UCP2 gene expression by a new β3-adrenoceptor agonist, trecadrine, in obese (cafeteria) rodents, but down-regulation in lean animals [DOI]
  38. 38 Mitochondrial factors in the pathogenesis of diabetes: a hypothesis for treatment.
  39. 39 Functioning of oxidative phosphorylation in liver mitochondria of high-fat diet fed rats [DOI]
  40. 40 Prenatal Effects of Different Intra-Uterine Milieus on Fetal Glucose Sensing Mechanisms during Gestation in Rats [DOI]
  41. 41 Maternal metabolic health and oocyte quality: the role of the intrafollicular environment
  42. 42 Transcriptional regulatory circuits controlling mitochondrial biogenesis and function [DOI]
  43. 43 Transcriptional feedback control of insulin receptor by dFOXO/FOXO1 [DOI]

Cited by 32

miR-138/miR-222 Overexpression Characterizes the miRNome of Amniotic Mesenchymal Stem Cells in Obesity

Carmela Nardelli, Ilaria Granata, Laura Iaffaldano · Stem Cells and Development · 2017

Enhanced mitochondrial biogenesis is associated with the ameliorative action of creatine supplementation in rat soleus and cardiac muscles

Mennatallah Gowayed, Shimaa Mahmoud, Yousria El‑Sayed · Experimental and Therapeutic Medicine · 2019

Transgenerational effects of obesity and malnourishment on diabetes risk in F2 generation

Mervat Y. Hanafi, Moustafa M. Saleh, Mohamed I. Saad · Molecular and Cellular Biochemistry · 2015

Maternal obesity and malnourishment exacerbate perinatal oxidative stress resulting in diabetogenic programming in F1 offspring

M. I. Saad, T. M. Abdelkhalek, M. M. Haiba · Journal of Endocrinological Investigation · 2015

In Utero Nutritional Manipulation Provokes Dysregulated Adipocytokines Production in F1 Offspring in Rats

Mervat Y. Hanafi, Mohamed I. Saad, Taha M. Abdelkhalek · Scientifica · 2016

Maternal and paternal obesity differentially reprogram the ovarian mitochondrial biogenesis of F1 female rats

Amina G. Ramadan, Wafaa M. Abdel-Rehim, Rasha A. El-Tahan · Scientific Reports · 2023

Showing 13 of 32 known citations — external sources report more than can currently be individually listed.

Article metrics

Real usage data collected on this platform.

48

Page views

0

PDF downloads

10

Outbound clicks

32

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.