In vitro Propagation of Oreocallis grandiflora (Lam.) R. Br., A Medicinal Threatened Plant
Percy Olivera-Gonzales, Edson M. Yldefonzo, Elder M. Mestanza, Carmen Tamariz-Angeles
Annual Research & Review in Biology · pp. 1–9 · Published 8 Jul 2017
10.9734/ARRB/2017/34724Abstract
Aims: To promote the conservation and biotechnology approach of Oreocallis grandiflora, a promising wild, medicinal and agroforestry Peruvian plant, a methodology for its in vitro propagation was performed. Study Design: Disinfection and germination of seed, followed by shoot multiplication and rooting using in vitro techniques. Place and Duration of Study: Follicles were collected in Yupa - Huaraz (9°30'26.76"S, 77°28'21.33"W) and the experimental procedure was done between January 2013 and December 2016. Methodology: Follicles with different colors were disinfected with ethanol 70% at different times, and seeds were place on basic culture medium composed by ½ Murashige and Skoog, sucrose (30 g·L-1) and phytagel (3 g·L-1), at pH 5.7. Shoot induction was performed using N6-benzylaminopurine (BAP) at 0.5, 1, 2, 3 and 4 mg·L-1. For root induction, α-naphthalenacetic acid (NAA) or gibberellic acid (GA3) at 0.25, 0.50, 0.75 and 1.0 mg·L-1 were used. Results: Disinfection of follicles at 15 and 20 min. of 70% ethanol were successful at 100%, and all seed germinated without addition of any growth regulator. The best shoot induction treatments were 3.0 and 4.0 mg·L-1 of BAP at 60 days of PGR exposition. For the root development PGR supplement was not necessary, but GA3 improved rooting percentage and explants growth while NAA induced callusing. Conclusion: This is the first report of in vitro propagation of O. grandiflora, and provides important results to promote its domestication, ex situ conservation, and biotechnological applications.
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Cited by 3
Carmen Tamariz-Angeles, Percy Olivera-Gonzales, Miguelina Santillán-Torres · Fungal Biology · 2023
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