Skip to content
Research Article Open access CC BY 3.0

Gene Encoding and Bioinformatics Analysis of Protein Structure of β-Galactosidase from Sunn Pest, Eurygaster integriceps Putton

Samin Seddigh, Maryam Darabi

Annual Research & Review in Biology · pp. 312–324 · Published 9 Oct 2013

10.9734/ARRB/2014/6194

Abstract

Aims: To identify the partial sequence of beta-galactosidase (EC 3.2.1.23) enzyme of sunn pest, Eurygaster integriceps Putton (Hemiptera: Scutelleridae), which is a key pest of wheat and barley in the wide area of the world and its relationship with other creatures. Place and Duration of Study: Department of Plant Protection, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran, and Department of Entomology, Science and Reaserch Branch, Islamic Azad University, Tehran, Iran. Between March 2012 and July 2013. Methodology: A part of β-galactosidase (βgal) gene was isolated from E. integriceps (designated as Ei-βgal-JQ889818), containing 328 bp. Nucleotide sequences were translated into 109 amino acids by translation tools. Twenty-six beta-galactosidase protein sequences from twenty-seven insect species, two animal samples including human and mouse, two bacteria samples including Escherichia coli and Synechococcus sp. and a sample of plants including Arabidopsis thaliana were aligned. Homology search was done by BLAST to identify the most similar protein sequences to Ei-βgal-JQ889818. Results: Protein structure analysis revealed that the deduced Ei-βgal-JQ889818 had extensive homology with other insect βgals and contained two catalytic domains of βgals. The predicted 3-D model of Ei-βgal-JQ889818 has a typical spatial structure of βgals and is partly similar to βgals. Phylogenetic tree analysis of Ei-βgal-JQ889818 showed that there is a close relationship among Arabidopsis thaliana, Acyrthosiphun pisum and Mus musculus. Conclusion: Accordingly, βgals should be functional proteins involved in the biosynthesis of lactose and are derived from a common ancestor. This research will lead us to know more about the role of βgal as a digestive enzyme through its phylogenetic relationship.

β-Galactosidase Carbohydrate enzyme Catalytic domain Eurygaster integriceps Phylogenetic tree.

References (18)

  1. 1 SWISS‐MODEL and the Swiss‐Pdb Viewer: An environment for comparative protein modeling [DOI]
  2. 2 Deviations from michaelis-menten behaviour of plant glutamate dehydrogenase with ammonium as variable substrate [DOI]
  3. 3 The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling [DOI]
  4. 4 Recent developments and future prospects in insect pest control in transgenic crops [DOI]
  5. 5 The structure of E. coli β-galactosidase [DOI]
  6. 6 Transgenic Plants for Insect Pest Control: A Forward Looking Scientific Perspective [DOI]
  7. 7 Small RNAs derived from longer non-coding RNAs [DOI]
  8. 8 Digestive amylase from the larger grain borer, Prostephanus truncatus Horn [DOI]
  9. 9 Digestive Proteolytic Activity in the Sunn Pest,Eurygaster integriceps [DOI]
  10. 10 Characterization of α-amylase in the midgut and the salivary glands of rice striped stem borer, Chilo suppressalis Walker (Lepidoptera: Pyralidae) [DOI]
  11. 11 Subcellular fractionation of midgut cells of the sunn pest Eurygaster integriceps (Hemiptera: Scutelleridae): Enzyme markers of microvillar and perimicrovillar membranes [DOI]
  12. 12 Economic Importance of Heteroptera: A General View [DOI]
  13. 13 Bioinformatics study of the 3-hydroxy-3-methylglotaryl-coenzyme A reductase (HMGR) gene in Gramineae [DOI]
  14. 14 Sunn pest, Eurygaster integriceps Putton (Hemiptera: Scutelleridae), digestive α-amylase, α-glucosidase and β-glucosidase [DOI]
  15. 15 The Amino Acid Sequence of β-Galactosidase [DOI]
  16. 16 Phylogenetic study of the 3-hydroxy-3-methylglotaryl-Coenzyme A reductase (HMGR) protein in six different family
  17. 17 Comparison of α- and β-mannosidase activity in the three cereal pests,Haplothrips triticiKurdjumov (Thysanoptera: Phlaeothripidae),Rhopalosiphum padiL. (Homoptera: Aphididae) andEurygaster integricepsPuton (Hemiptera: Scutelleridae) [DOI]
  18. 18 Comprehensive Molecular Insect Science [DOI]

Cited by 1

Partial gene identification and protein analysis of β-galactosidase from bird cherry-oat aphid

S. Seddigh · 2018

Related research

Article metrics

Real usage data collected on this platform.

28

Page views

0

PDF downloads

3

Outbound clicks

1

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.