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Research Article Open access CC BY 3.0

Fermenation Products of β-Mannanase Producing Bacteria Inhibit Selected Poultry Borne Pathogens

O. O. Olaniyi, D. J. Arotupin

Microbiology Research Journal International · pp. 612–622 · Published 27 Aug 2013

10.9734/BMRJ/2013/4220

Abstract

Aim: The study evaluated the inhibitory effect of fermentation products of β-mannanase-producing bacteria on selected poultry borne pathogens. Study Design: The first experiment, bacterial isolates previously confirmed positive for mannanase by plate assay technique were further screened for mannanase production in submerged state fermentation. In the second experiment, inhibitory effect of fermentation products of mannanase-producing bacteria on selected poultry pathogens was evaluated. Place and Duration of Study: Microbiology Research Laboratory, Federal University of Technology, Akure Nigeria between September 2011 and March 2012. Methodology: Bacterial isolates from agricultural wastes previously confirmed positive for mannanase activity by plate assay were further screened for their potential performance under submerged state fermentation and enzyme activity determined by dinitrosalicylic acid method. The inhibitory action of β-mannanase-producing bacteria was determined by supplementation of supernatant and plating method. Results: Isolate 1A showed highest mannanase activity (13.430 U/ml), displayed broad inhibition to selected poultry borne pathogens; Klebsiella oxytoca, Shigella alkalescens, Escherichia coli, Salmonella typhii, Staphylococcus aureus and Streptococcus sp. Apart from isolate 1A, fermentation products of other isolates generated from the mannolytic action of β-mannanase on mannan containing substrate displayed different percentage inhibition on selected poultry borne pathogens. Conclusion: The results suggested that fermentation products from β-mannanase-producing bacteria might possess antibacterial properties which could be applied in poultry farms.

β-mannanase pathogens percentage inhibition fermentation products.

References (21)

  1. 1 Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar [DOI]
  2. 2 Lignocellulosic residues: Biodegradation and bioconversion by fungi [DOI]
  3. 3 Alternatives to antibiotics: bacteriocins, antimicrobial peptides and bacteriophages [DOI]
  4. 4 Characterization of cellulases and hemicellulases produced by Trichoderma reesei on various carbon sources [DOI]
  5. 5 High-level production, purification and characterization of a thermostable β-mannanase from the newly isolated Bacillus subtilis WY34 [DOI]
  6. 6 Ribosomally Synthesized Antibacterial Peptides in Gram Positive Bacteria [DOI]
  7. 7 Genetically Engineered Bacteriocins and their Potential as the Next Generation of Antimicrobials [DOI]
  8. 8 Biobleaching of wheat straw-rich soda pulp with alkalophilic laccase from γ-proteobacterium JB: Optimization of process parameters using response surface methodology [DOI]
  9. 9 Purification and characterization of a β-mannanase from Trichoderma harzianum strain T4 [DOI]
  10. 10 Bleaching of wheat straw-rich soda pulp with xylanase from a thermoalkalophilic Streptomyces cyaneus SN32 [DOI]
  11. 11 Production of -mannanase by Bacillus amylolequifaciens 10A1 cultured on potato peels [DOI]
  12. 12 Optimization of xylanase and mannanase production by Bacillus circulans strain BL53 on solid-state cultivation [DOI]
  13. 13 Optimization of medium composition for the production of alkaline β-mannanase by alkaliphilic Bacillus sp. N16-5 using response surface methodology [DOI]
  14. 14 Solid state fermentation and characterization of partially purified thermostable mannanase from Bacillus sp. MG-33 [DOI]
  15. 15 Bioconversion of Selected Agricultural Wastes and Associated Enzymes by Volvariella volvacea: An Edible Mushroom [DOI]
  16. 16 Enhancing α-Amylase and Cellulase in vivo Enzyme Expressions on Sago Pith Residue Using Bacilllus amyloliquefaciens UMAS 1002 [DOI]
  17. 17 Isolation, Screening and Identification of Mannanase Producing Microorganisms
  18. 18 Optimization of cellulase and -glucosidase induction by sugarbeet pathogen Sclerotium rolfsii [DOI]
  19. 19 Chryseobacterium indologenes, novel mannanase-producing bacteria
  20. 20 Bergey's Manual of Determinative Bacteriology [DOI]
  21. 21 Bergey's Manual of Determinative Bacteriology [DOI]

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