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Research Article Open access CC BY 3.0

Development of a New Eco-Friendly Validated UV Spectrophotometric Method for Quantitative Determination of Acetaminophen in Tablet Dosage Form

Swati Jain, Ashutosh Pareek, Sarita singh, Pratima ., Sangeeta Kumari, Seema Verma, Vivek Jain, Yashumati Ratan

International Research Journal of Pure and Applied Chemistry · pp. 181–189 · Published 13 Nov 2013

10.9734/IRJPAC/2014/5784

Abstract

Aims: The present study was undertaken to develop a new UV spectroscopic method of acetaminophen using mix solution of methanol: phosphate buffer 7.4 (in 1:9 ratio) (further diluted with phosphate buffer 7.4 only) as a solvent system for analysis of acetaminophen. Place and Duration of Study: Department of Pharmacy, Banasthali University, Rajasthan between July 2012 and August 2012. Methodology: Method was developed and various validation parameter as per ICH Q2B guideline were tested like LOD, LOQ, precision, molar absoptivity, sandells’ sensitivity and stability testing for the assessment of newly developed method. Furthermore method was also compared with already existed official method using pure methanol (Class II) as solvent. Finally marketed acetaminophen tablets were subjected for determination of percent purity and recovery test in three different levels (80%, 100% and 120%) using the developed method. Results: The λ max (absorption maxima) of the drug was found to be 247 nm. A linear response was observed in the range of 0-45μg/ml with a regression coefficient of 0.997. Beside this solution was significantly stable for 15 days. The percent purity (99.09%) and % recovery at 80, 100 and 120 % were found to be 99.89, 99.00, and 100.02 respectively. Conclusion: Result suggested that the newly developed method having significant similar data irrespective of decrease in methanol used, which revealed that new developed method is a simple, accurate, precise, specific, sensitive, cost effective, reproducible and an eco-friendly alternative for UV spectrophotometric determination of acetaminophen.  

Acetaminophen spectroscopic molar absorptivity sandell’s sensitivity

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