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Research Article Open access CC BY 3.0

Characterization of the Biochemical Pathway of Apoptosis Induced by D-glucopyranoside Derivatives from Tulbaghia violacea

Sylvester Leonard Lyantagaye

Annual Research & Review in Biology · pp. 962–977 · Published 13 Dec 2013

10.9734/ARRB/2014/6158

Abstract

Aim: Chinese hamster ovary (CHO) cells were used to identify the most likely apoptotic biochemical pathway induced by Methyl-α-D-glucopyranoside (MDG), D-fructofuranose-β(2→6)-methyl-α-D-glucopyranoside (DFMDG) and β-D-fructofuranosyl-(2→6)-α-D-glucopyranoside (DFDG) treatments isolated from wild garlic (Tulbaghia violacea). Methodology: Assays, which detect different morphological and biochemical changes in the CHO cells were used to examine the apoptosis induction by individual agents, paying special attention to the mitochondria membrane potential (∆ψm) depolarization. Results: The major events of apoptosis, namely, translocation of phosphatidylserine, mitochondria membrane potential (∆ψm) depolarization and activation of caspase-3 in CHO cells were initiated within 2 hours of treatments. The onset of the apoptosis induction was indistinguishable among the MDG, DFMDG and DFDG, and similar to that of staurosporine. Conclusion: The study shows that CHO cells treated with MDG, DFMDG and DFDG undergo apoptotic death through the mitochondrial (intrinsic) pathway.

Apoptosis mitochondrial pathway glucopyranosides mitochondrial permeability transition pore

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