Skip to content
Research Article Open access CC BY 4.0

Bacteriological and Physicochemical Profiles of Soils in Selected Oil-Contaminated Sites in Yorla, Ogoni Land

Onyinyechi Stainless Uzor, Gideon Chijioke Okpokwasili, Ejiro Obakpororo Agbagwa

Microbiology Research Journal International · pp. 104–117 · Published 28 Oct 2020

10.9734/mrji/2020/v30i830256

Abstract

Bacteriological and physicochemical properties of oil–polluted sites in Yorla, Ogoniland was studied. Soil samples were collected from three different points and analyzed for bacteriological and physicochemical characterization using standard methods. The total culturable heterotrophic bacteria (THC) counts from the polluted sites ranged from 7.2. *107to9.5*107cfu/g while the THC of the unpolluted sites ranged from 11.9 *107 to 12.9 *107cfu/g. Hydrocarbon utilizing bacterial (HUB) counts from the polluted sites ranged from 2.8 *106 to 3.3 * 106cfu/g while the hydrocarbon utilizing bacterial (HUB) counts of the unpolluted sites ranged from 1.1 *106to 1.5 *106.Bacteria obtained from the various sites were isolated using standard microbiological methods. Identification of isolates was carried out using 16S rRNA (Ribonucleic acid) gene amplification and sequencing. Electropherograms of generated sequences were inspected with Chromas Lite 2.0.1 software. Sequence identification was carried out using GenBank’s Basic Local Alignment Search Tool (BLAST) algorithm of National Centre for Biotechnology and Information (NCBI). Sequence analysis revealed the presence of Acinetobacter venetianus , Bacillus cereus, Bacillus flexus, Bacillus thuringiensis, Acinetobacter junii, Shewanella sp., Lysinibacillus xylanilyticus and Pseudomonas pseudoalcaligenes,  The physicochemical properties of the soil samples analyzed showed that the pH values of the polluted sites ranged from 7.86 to 9.16 while those of  the unpolluted sites were 6.19 and 7.10. Total organic carbon (TOC) contents of the polluted sites ranged from 3.18% to 3.96% while the unpolluted sites TOC ranged from 3.00% -3.06%. Statistical analyses of the results were carried out using analysis of variance (ANOVA) and t-test analysis. This work revealed the presence of reasonable population of indigenous hydrocarbon-utilizing bacteria in oil-polluted sites in Yorla community which can be monitored and enhanced to improve their bioremediation abilities in the oil-polluted sites.

Hydrocarbon-pollution Niger Delta Biodegradation Hydrocarbon utilizers.

References (15)

  1. 1 The neighbor-joining method: a new method for reconstructing phylogenetic trees. [DOI]
  2. 2 CONFIDENCE LIMITS ON PHYLOGENIES: AN APPROACH USING THE BOOTSTRAP [DOI]
  3. 3 Microbial degradation of hydrocarbons in the environment [DOI]
  4. 4 Microbial Degradation of Petroleum Hydrocarbon Contaminants: An Overview [DOI]
  5. 5 Marine microorganisms make a meal of oil [DOI]
  6. 6 Obligate oil-degrading marine bacteria [DOI]
  7. 7 Microbial Population Changes during Bioremediation of an Experimental Oil Spill [DOI]
  8. 8 Microbial Activity and Community Composition during Bioremediation of Diesel-Oil-Contaminated Soil: Effects of Hydrocarbon Concentration, Fertilizers, and Incubation Time [DOI]
  9. 9 Petroleum degrading potentials of single and mixed microbial cultures isolated from rivers and refinery effluent in Nigeria [DOI]
  10. 10 Bacterial Communities from Shoreline Environments (Costa da Morte, Northwestern Spain) Affected by the Prestige Oil Spill [DOI]
  11. 11 Bioremediation of Petroleum Pollutants [DOI]
  12. 12 Degradation of straight-chain aliphatic and high-molecular-weight polycyclic aromatic hydrocarbons by a strain of Mycobacterium austroafricanum [DOI]
  13. 13 Effects of Refined Petroleum Hydrocarbon on Soil Physicochemical and Bacteriological Characteristics [DOI]
  14. 14 Nitrogen fixing bacteria enhanced bioremediation of a crude oil polluted soil [DOI]
  15. 15 Evaluation of bioremediation effectiveness on sediments Contaminated with industrial wastes

Cited by 5

Evaluating effect of aeration and sand addition on soil physicochemical properties and soil total petroleum hydrocarbon bioremediation efficiency by indigenous isolated bacteria

Parviz Behdarvandan, Reza Jalilzadeh Yengejeh, S. Sabzalipour · Toxicological & Environmental Chemistry · 2024

Bioremediation of heavy metals polluted environment and decolourization of black liquor using microbial biofilms

I. Liaqat, Noor Muhammad, C. Ara · Molecular Biology Reports · 2023

Isolation and Molecular identification of Phenol tolerating bacteria from petroleum contaminated sites

S. M. Dankaka, N. Abdullahi · Bayero Journal of Pure and Applied Sciences · 2022

Showing 3 of 5 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

84

Page views

0

PDF downloads

11

Outbound clicks

5

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.