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Antisalmonella Activity and Modes of Action of Extracts, Fractions and Cerevisterol from Impatiens burtonii against Multi-Drug Resistant Salmonella

Aurelie Dahlia Yemeli Piankeu, Frank Wilson Tiatsop Kenmoe, Aimé Gabriel Fankam, Michel Noubom, Siméon Pierre Chegaing Fodouop, Rémy Bertrand Teponno, Léon Azefack Tapondjou, Donatien Gatsing

Microbiology Research Journal International · pp. 54–71 · Published 22 Jun 2024

10.9734/mrji/2024/v34i71458

Abstract

Background: Typhoid fever is an endemic disease in developing countries, especially throughout Asia and Africa, which may lead to life-threatening conditions in the absence of adequate treatment. Objective: This study was aimed at determining the in vitro activity of the crude extracts, fractions and compounds of Impatiens burtonii against drug sensitive and multidrug-resistant Salmonella strains. Methods: Moreover, the modes of action of the most active compound, cerevisterol (1) were investigated on Salmonella TyphiATCC6539.The in vitro antisalmonella activity of the samples was evaluated by the broth microdilution method. Catalase Activity was done by measuring the high of the foam in the presence of hydrogen peroxide. Bacteriolysis, time kill-assay, nucleic acid and protein leakage potential of cerevisterol were carried out through spectrophotometric methods. Finally, time kill-assay and proton-ATPase pump activity were determined by bacterial count and pH measurement, respectively. Results: The In vitro antisalmonella activity revealed that the hydroethanolic 75% crude extract of Impatiens burtonii showed the highest antisalmonella activity, with minimal inhibitory concentration (MIC) ranging from 32 to 512 μg/mL. Among the fractions of that extract, the dichloromethane (DM) fraction exhibited the highest activity, with MIC ranging from 16 to 32 μg/mL. Cerevisterol (1) isolated from the DM extract was the most active compound (8 ≤ MIC≤ 128 μg/mL). This study showed that cerevisterol is bacteriostatic against Salmonella Typhi. Moreover, cerevisterol induced the release of the nucleotides, inhibited H+-ATPase proton pumps, and reduced the catalase activity of Salmonella Typhi ATCC6539. Conclusion: Finally, this study allowed to identify cerevisterol as a good candidate to tackle typhoid fever infections and mainly that caused by MDR (multidrug-resistant) strains.

Typhoid fever Impatiens burtonii cerevisterol antisalmonella activity mode of action

References (18)

  1. 1 A Sensitive and Quick Microplate Method to Determine the Minimal Inhibitory Concentration of Plant Extracts for Bacteria [DOI]
  2. 2 The role and place of medicinal plants in the strategies for disease prevention [DOI]
  3. 3 Plant Secondary Metabolites in the Battle of Drugs and Drug-Resistant Bacteria: New Heroes or Worse Clones of Antibiotics? [DOI]
  4. 4 Typhoid vaccines: WHO position paper.
  5. 5 Antibacterial, antifungal and antitubercular activity of (the roots of) Pelargonium reniforme (CURT) and Pelargonium sidoides (DC) (Geraniaceae) root extracts [DOI]
  6. 6 Ethnopharmacological survey of plants used for the treatment of female infertility in Baham, Cameroon [DOI]
  7. 7 Typhoid Fever Surveillance, Incidence Estimates, and Progress Toward Typhoid Conjugate Vaccine Introduction — Worldwide, 2018–2022 [DOI]
  8. 8 The phylogeography and incidence of multi-drug resistant typhoid fever in sub-Saharan Africa [DOI]
  9. 9 Antibacterial and antibiotic resistance modulatory activities of leaves and bark extracts of Recinodindron heudelotii (Euphorbiaceae) against multidrug-resistant Gram-negative bacteria [DOI]
  10. 10 Antimicrobial resistant strains of Salmonella typhi: The role of illicit antibiotics sales, misuse, and self-medication practices in Pakistan [DOI]
  11. 11 Medicinal plants used by Burundian traditional healers for the treatment of microbial diseases [DOI]
  12. 12 Salmonella Typhimurium Infection Leads to Colonization of the Mouse Brain and Is Not Completely Cured With Antibiotics [DOI]
  13. 13 Methanol extract from the seeds of Persea americana displays antibacterial and wound healing activities in rat model [DOI]
  14. 14 Isolation, characterization and in vitro anti-salmonellal activity of compounds from stem bark extract of Canarium schweinfurthii [DOI]
  15. 15 A Salmonella Typhi Controlled Human Infection Study for Assessing Correlation between Bactericidal Antibodies and Protection against Infection Induced by Typhoid Vaccination [DOI]
  16. 16 Impatiens banen and Impatiens etugei (Balsaminaceae), new threatened species from lowland of the Cross-Sanaga Interval, Cameroon [DOI]
  17. 17 Innate Immunity in Health and Disease [DOI]
  18. 18 In vitro Antioxidant Activities and Effect of Hydroethanolic and Aqueous Extracts of Terminalia avicennioides (Combretaceae) on Salmonella [DOI]

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