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Richard Owusu-Apenten

Publications (9)

Determination of Iron (III) Reducing Antioxidant Capacity for Manuka Honey and Comparison with ABTS and Other Methods

Hasif Ilyasa Mohd Yusof, Richard Owusu-Apenten & Poonam Singh Nigam · Journal of Advances in Biology & Biotechnology · 2018

Aims: Applying multiple assays with trolox as the sole reference compound is a recent AOAC proposal to improve the reliability of total antioxidant capacity determinations. The aim of this study was to evaluate, iron (III) reducing antioxidant capacity (iRAC) for Manuka honey sam...

Open access Research Article 10.9734/JABB/2018/42202

Total Phenols, Antioxidant Capacity and Antibacterial Activity of Manuka Honey Extract

Tsz Ching Chau, Richard Owusu-Apenten & Poonam Singh Nigam · Journal of Advances in Biology & Biotechnology · 2017

Total Phenols, Antioxidant Capacity and Antibacterial Activity of Manuka Honey Extract Aims: To evaluate total phenols content (TPC), antioxidant capacity (TAC) and antibacterial activity of Manuka honey extract (MHE) and to compare such properties with those for unfractionated M...

Open access Research Article 10.9734/JABB/2017/37101

Effects of Ascorbic Acid, Dehydroascorbic Acid and Methotrexate on Breast Cancer Cell Viability

Yewande Dosunmu & Richard Owusu-Apenten · Journal of Applied Life Sciences International · 2017

Aims: To examine the effects of ascorbic acid (AA), dehydroascorbic acid (DHA) and methotrexate (MTX) combined treatments on (MDA-MB-231) breast cancer cell viability and intracellular reactive oxygen species (ROS). Study Design: In-vitro method. Place and Duration of Study: Biom...

Open access Research Article 10.9734/JALSI/2017/37185

Methotrexate Combination Effects with Genistein and Daidzein on MDA-MB-231 Breast Cancer Cell Viability

Ashleigh Maginnes & Richard Owusu-Apenten · Journal of Applied Life Sciences International · 2017

Aims: To investigate the effect of methotrexate and soy isoflavones genistein and daidzein on MDA-MB-231 breast cancer cell viability. Study Design: In-vitro study using cultured cells Place and Duration of Study: Nutrition Innovations Centre for Food & Health (NICHE), School...

Open access Research Article 10.9734/JALSI/2017/35206

Effect of Iron and Hydrogen Peroxide Supplementation on the Total Phenols Content and Cytoxicity of Honey for MCF-7 Breast Cancer Cells

Lai Yung Wong, Poonam Singh Nigam & Richard Owusu-Apenten · Journal of Advances in Biology & Biotechnology · 2018

Aims: To evaluate the effect of supplementation with iron (II/III) salts and/ or hydrogen peroxide on Manuka honey total phenols content (TPC) and the cytoxicity toward MCF-7 breast cancer cells. Study Design:  The study had an In Vitro design. Place and Duration of Study: Ulster...

Open access Research Article 10.9734/JABB/2018/42344

Total Phenols, Antioxidant Capacity and Antibacterial Activity of Manuka Honey Chemical Constituents

Graeme Kirkpatrick, Poonam S. Nigam & Richard Owusu-Apenten · Journal of Advances in Biology & Biotechnology · 2017

Aims: To compare the total phenol content, antioxidant capacity and antibacterial activity of methyl syringate (MSY), methylglyoxal (MGO) and phenyllactic acid (PLA) as major components from Manuka honey Study Design: In-vitro study. Place and Duration of Study: Nutrition Innovat...

Open access Research Article 10.9734/JABB/2017/37242

A Combination of Curcumin from Turmeric and Alpha-linolenic Acid Shows Antagonism with MCF-7 Breast Cancer Cells in Phenol-red Free Medium

Tahrir Aldhirgham, Kathleen Henderson, Poonam S. Nigam & Richard Owusu-Apenten · Journal of Applied Life Sciences International · 2016

Aims: To determine the total phenols content and antioxidant capacity for turmeric and curcumin, and to assess the effect of alpha-Linolenic acid (ALA) combinations treatments on MCF-7 breast cancer cell viability and intracellular reactive oxygen species (ROS). Study Design: In-...

Open access Research Article 10.9734/JALSI/2017/29740

Evaluation of Manuka Honey Estrogen Activity Using the MCF-7 Cell Proliferation Assay

Kathleen Henderson, Tahrir Aldhirgham, Poonam Singh Nigam & Richard Owusu-Apenten · Journal of Advances in Biology & Biotechnology · 2016

Aims: To assess the estrogenic activity of Manuka honey using the MCF7 cell proliferation assay. Study Design:  In vitro cell based E-screen. Place and Duration of Study: Ulster University, Coleraine, UK, September 2015 to September 2016. Methodology: Manuka honey (UMF15+) was ch...

Open access Research Article 10.9734/JABB/2016/29887

Effect of pH on the Radical Quenching Capacity of Tea Infusions Using the ABTS•+ Assay

Yuk Man Chan, Nga Kwan Cheng, Poonam Singh Nigam & Richard Owusu-Apenten · Journal of Applied Life Sciences International · 2016

Aims: The aims of this study were to assess the impact of pH on the free radical quenching activity of tea infusions using a modified 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assay and three antioxidant compounds as reference. Study Design: In-vitro method....

Open access Research Article 10.9734/JALSI/2016/27235