Richard James

Optimization and Evaluation of Triplex Real-time PCR Assay for Detection of Genes Encoding Staphylococcal Virulence and Methicillin Resistance Using Two Different Multi-channel Emission Instruments

Charles Emeka Okolie & Richard James · Journal of Applied Life Sciences International · 2015

Aim of Study: To optimize a triplex real-time PCR assay developed elsewhere and to evaluate the performance characteristics of two different multi-channel real-time PCR systems on the newly optimized assay. Methodology: A triplex real-time PCR assay developed for three key genes...

Open access Research Article 10.9734/JALSI/2015/12939

Development of New Pentaplex PCR Assay for Differentiating Staphylococci from Other Bacteria with Simultaneous Detection of Staphylococcus aureus Genes Encoding Panton-Valentine Leukocidin and Methicillin Resistance

Charles Emeka Okolie & Richard James · Journal of Advances in Biology & Biotechnology · 2015

In some human infections including those of blood, skin and respiratory tract, the causative bacterial agents tend to overlap, especially Staphylococcus spp. and Streptococcus spp. Such overlaps constitute difficulties in the choice of diagnostic tools, antibacterial chemotherapy...

Open access Research Article 10.9734/JABB/2015/12934