Leelavathy Nanjappa

Separation and Optimisation of a Sucrose Density Gradient Centrifugation Protocol for Isolation of Peripheral Blood Mononuclear Cells (PBMC)

Sudeep Nagaraj, Shubha Nivargi, Leelavathy Nanjappa & Jagadish Tavarekere Venkataravanappa · Asian Journal of Research in Biochemistry · 2018

One step centrifugation procedure used commonly for separation of blood cells is the ficoll gradient centrifugation. In this method, after centrifugation, the peripheral blood mononuclear cells (PBMCs) are located on the top of the separation fluid, whereas other blood cells eryt...