Comparison of Medium Supplementation on Proliferation and Hormone Production of Bovine Granulosa Cells in a Defined Culture System
L. H. Montrezor, C. A. Piccinato, C. V. A. Collares, A. A. Vireque & A. A. M. Rosa e Silva · Journal of Scientific Research and Reports · 2013
Aims: The objective of this study was to develop a serum-free bovine granulosa cell (GC) culture system in which estradiol (E2) production could be maintained in a defined media, with polyvinyl alcohol (PVA), insulin and insulin-like growth factor-1 (IGF-1) and without FSH and co...