Optimization of Real-time Reverse Transcriptase Polymerase Chain Reaction for Detection of Dengue Virus
Kaunara A. Azizi, Arnold J. Ndaro, Athanasia Maro, Adonira Saro & Reginald A. Kavishe · Journal of Advances in Microbiology · 2020
Aims: This study was set to optimize conditions for real time reverse transcriptase polymerase chain reaction (RT-PCR) for detection of dengue virus by using rapid and simple nucleic acid extraction method. Methodology: One step and two step real time RT-PCR were evaluated in dif...
Open access
Research Article
10.9734/jamb/2020/v20i830269