Abhinandan Ravsaheb Patil

Simultaneous Extraction, Purification, and Quantification of Polyphenols, Caffeine, and Theophylline from BB35 Green Tea Leaves (Kenya cultivar) Using High-Performance Liquid Chromatography (HPLC) with Gradient Quantification

Hemanth Kumar Manikyam, Cecilliah N Ngugi, Fridah Marete, Elizabeth Kagotho, Sandeep Balvant Patil & Abhinandan Ravsaheb Patil · South Asian Research Journal of Natural Products · 2024

This study introduces a novel technique that uses a solvent mixture of 60% water and 40% ethanol to extract caffeine, theophylline, and polyphenols from green tea leaves (Camellia sinensis) all at once. On a dry basis, the Folin-Ciocalteu Reagent Method (ISO 14502-1) estimated th...